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. Author manuscript; available in PMC: 2009 Jul 4.
Published in final edited form as: J Mol Biol. 2008 May 2;380(2):303–312. doi: 10.1016/j.jmb.2008.04.061

Figure 2. The function of ING1 requires its H3K4me3 binding activity.

Figure 2

(a) Assessment of the repair rate of UV-damaged DNA by luciferase reporter assay in MMRU cells cotransfected with undamaged or damaged pRL-CMV luciferase plasmid and pcDNA3-vector (control), wild-type ING1 expression vectors and ING1W235A mutant. Forty hours after transfection, luciferase activity was measured with a luminometer. Columns, mean from triplicates; bars, SD. The experiment was repeated twice with similar results. (b) Stimulation of apoptosis by overexpressed full-length wild type ING1 and W235A mutant. Cell death was measured in HT1080 cells transfected with 1 µg of the indicated expression vectors. The cells were first treated with Doxorubicin and then trypsinized and stained with trypan blue. The error bars represent the mean ± s.e.m. from three independent experiments.