Figure 1.

The Dictyostelium lvsA locus. The lvsA locus was mutated by REMI. The * denotes the site of insertion of plasmid pRHI30 used for REMI. Probe A is the portion of the lvsA gene that was cloned from the REMI mutant AD60. A larger fragment was used to design a knockout construct (KO construct) using plasmid pRHI30 as a transformation vector. The letters KO on the lvsA locus indicate the site of plasmid insertion in the knockout strains. The lvsA ORF is interrupted by three small introns at the positions indicated. Restriction sites indicated are the following: A, AccI; B, BamHI; Bg, BglII; E, EcoRI; N, NdeI; and S, SphI.