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. 1999 Dec;10(12):4429–4439. doi: 10.1091/mbc.10.12.4429

Figure 2.

Figure 2

Dictyostelium lvsA mutants are defective in cytokinesis. (A) Southern blot analysis of genomic DNA from Dictyostelium cells is shown. The DNA was digested with EcoRI, and the blot was probed with probe A (Figure 1). This probe detects a 1.5-kb EcoRI band in DNA from wild-type (DH1) and control (AD59) cells. This band is replaced by a 6-kb band in the lvsA REMI mutant (AD60) and knockout strains (AD61–AD63). (B) LvsA mutants cannot grow in suspension culture. The different strains were placed in suspension culture in nutrient-rich medium. Wild-type and control cells grow at fast rates under these conditions. In contrast, the lvsA REMI mutant (AD60) and knockout strains (AD61 and AD63) fail to grow. (C) LvsA mutants fail in cytokinesis. Cells were fixed after being in suspension for 2 d and were stained with DAPI. Most wild-type (DH1) cells have one or two nuclei because they divide normally in suspension. The lvsA-mutant strains (AD60 and AD63) accumulate numerous nuclei because they fail to divide.