Figure 7.

Pinocytosis in lvsA and clathrin-null mutants. Endocytosis of rhodamine-labeled dextran was determined as described (Hacker et al., 1997). Clathrin-null cells have a strong defect in their ability to internalize this fluid phase marker. LvsA-mutant cells can endocytose fluid but at a slightly reduced rate compared with wild-type cells. Each data point is the average of duplicate measurements. Symbols indicate the following: ○, wild-type DH1 cells; ▪, clathrin-null cells; □, lvsA REMI mutant AD60; and ▴, lvsA knockout mutant AD63.