Figure 8.

Capping of cell surface receptors in lvsA and clathrin-null mutants. Different Dictyostelium strains were challenged with FITC-labeled ConA to determine their capping response. (A) Wild-type (DH1) cells were able to form very tight caps within 5 min. (B) Clathrin-null cells formed caps, but only to a limited extent. (C and D) LvsA-mutant strains AD60 (C) and AD63 (D) also formed caps with a C shape similar to that formed by the clathrin mutants. Incubation time for all three mutant strains was 30 min.