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FasL mRNA induction after TCR activation. Fractions of elutriated and nonelutriated CTL 1520 (A) and Jurkat cells (B) were plated on plates precoated with OKT-3 mAb (+) or control immunoglobulin G (−) for 2.5 h, after which total mRNA was extracted for analysis by an RNase protection assay. FasL- and glyceraldehyde-3-phosphate dehydrogenase (GAPDH)-protected bands are shown. Data are representative of at least two independent experiments.
