Figure 5.

Induction of apoptosis by incubation with soluble FasL or by TCR activation. Elutriated and nonelutriated CTL 907 were plated with (B) or without (A) 50 ng/ml soluble rFasL and 1 μg/ml enhancer or on plates precoated with OKT-3 mAb (C). After 5 h cells were collected and stained with Annexin-V-FITC (x-axis) and 7-AAD (y-axis) to discriminate between viable (double-negative), apoptotic (Annexin-V-positive, 7-AAD-negative) and necrotic (double-positive) cells. Results are representative of two independent experiments.