Figure 4. Cross-hybridization of gene- and species-specific probes and ILP-PCR products.

Alexa Fluor® 532-labeled ILP-PCR products from 3 bacterial species were incubated with gene-specific probes from different bacterial origins for 20 minutes. The fluorescence signals were then scored using a suspension array flow analyzer. Results for six of the tested genes are shown. In some experiments, data from only 3 genes are presented due to the unavailability of the gene sequences.