Figure 7.

Single-channel conductances in α1β2γ2S 1:1:1 transfections. A, Single-channel openings in tail currents after deactivation from a 200 ms, 10 mm GABA pulse with openings indicative of αβγ receptors (e.g., 29 pS) or αβ receptors (e.g., 10 pS, inset). B, Clusters of single openings from an excised patch held at ± 80 mV. Amplitude histograms (center) of the closed and open levels for the clusters indicated (arrows) and Gaussian fits yielding ∼15 pS chord conductance are shown, consistent with αβ receptors. C, Current–voltage plots were constructed for each of the transfections (right), culled from seven patches from six different transfections in which single-channel openings were detectable. Calculated chord conductances are listed beneath the plot for main and subconductances (sub). Data are mean ± SD.