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. Author manuscript; available in PMC: 2009 Oct 10.
Published in final edited form as: Mol Cell. 2008 Oct 10;32(1):11–20. doi: 10.1016/j.molcel.2008.09.011

Figure 2.

Figure 2

Over-expression of SIRT1 inhibits growth of Brca1Co/Co; MMTV-Cre;p53+/− tumor cells. (A, B) Over-expression of SIRT1 inhibits the allografted tumor growth of Brca1Co/Co;MMTV-Cre;p53+/− tumor cells (cell line: 69) compared with GFP transfected cells as reflected by average tumor volume (mm3) ±SE (A) and average weight (mg) ±SE (B). The tumor size was measured daily starting one week post transplantation, and the tumor weight was recorded when animals were sacrificed. P is a student t-test value and represents the comparison between the GFP and SIRT1 tranfected tumors. (C–E) Over-expression of SIRT1 reduced growth of 69 cells (C) and induced cell death at the same time. Cell death at day 6 is shown. (E). (D) Western blot to measure the expression level of SIRT1 in SIRT1 transfected 69 cells at 1 to 12 days after sorting. (F, G) Over-expression of SIRT1 did not inhibit the allografted tumor growth of MMTV-Neu;p53+/−tumor cells (cell line: Neu) compared with GFP transfected cells by volume (F) and weight (G). (H–J) Over-expression of SIRT1 did not affect the growth (H) and cell death of Neu cells. Cell death at day 6 is shown. (J). (I) Western blot analysis to monitor the expression of SIRT1 in SIRT1 transfected Neu tumor cells at 1 to 12 days after sorting. For the nude mice study, each group of data was collected from 10 individual mice, bearing 2 inoculated tumors each. Data is presented as average±SD if it is not specifically mentioned.