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. Author manuscript; available in PMC: 2009 Oct 10.
Published in final edited form as: Mol Cell. 2008 Oct 10;32(1):11–20. doi: 10.1016/j.molcel.2008.09.011

Figure 3.

Figure 3

Resveratrol inhibits Brca1Co/Co;MMTV-Cre;p53+/− tumor cell growth in vitro and in allograft tumors. (A, B) Resveratrol reduces Brca1Co/Co;MMTV-Cre;p53+/− tumor cell colony formation in soft agar in both size (A) and number (B). (C, D) Resveratrol does not affect MMTV-Neu;p53+/− tumor cell colony formation in soft agar in both size (C) and number (D). In (B) and (D), data are average ± SD. (E) Nude mice were pretreated with carrier (control), 5μg, or 10μg revesveratrol per 30g body weight daily by IP injection for 10 days. The Brca1Co/Co;MMTV-Cre;p53+/− tumor cells were implanted subcutaneously and resveratrol treatment was provided daily. The tumor size was measured daily starting 1-week post transplantation (E). Data shown is mean ±SE. The tumor weight was recorded when animals were sacrificed (F). Data is Mean±SE. P is a Student t-test value and represents the comparison between the control and two treated groups. (G, H) Resveratrol treatment did not inhibit allografted MMTV-Neu;p53+/− tumor cells in volume (G) and weight (H). In (G) and (H), data are Mean±SE. (I) Brca1Co/Co;MMTV-Cre;p53+/− tumor cells were innoculated subcutaneously into nude mice and resveratrol treatment started at 5μg/30g either next day (Day1+Res) or until the tumor was visible (Tumor+Res). For all in vivo resveratrol treatment, data for each group of allografted tumors was collected from 10 individual nude mice, bearing 2 tumors each. The data is presented as Mean±SE.