Table 1.
Complete list of LC/MS total ion abundance ratios and percentages for all connective tissue samples. Ratios and percentages are shown as the average of triplicate runs of each tissue sample.
| Tissue Sample | Δ-unsaturated/saturated | % TIA/μg DNAa | % Non- reducing end terminal HexAb | % Under- sulfated Δ-unsaturatedc | % unsulfated linkerd |
|---|---|---|---|---|---|
| JB-Cartilage A1 | 7.7 ± 0.6 | N/A | 75.5 ± 4.0 | 11.9 ± 0.9 | 61.0 ± 7.1 |
| JB-Cartilage B1 | 2.7 ± 0.1 | 2.8 | 76.1 ± 1.7 | 9.1 ± 0.5 | 61.9 ± 2.8 |
| JB-Cartilage B2 | 6.9 ± 0.6 | 2.1 | 80.2 ± 1.1 | 7.5 ± 0.5 | 59.6 ± 1.8 |
| JB-Cartilage B3 | 2.8 ± 0.1 | 2.3 | 48.9 ± 0.9 | 17.2 ± 0.5 | 73.3 ± 1.3 |
| H- Cartilage A2 | 1.9 ± 0.1 | 13.7 | 74.9 ± 1.6 | 6.4 ± 0.4 | 66.4 ± 2.7 |
| H- Cartilage B1 | 2.3 ± 0.1 | 18.5 | 69.6 ± 4.9 | 4.7 ± 0.3 | 33.2 ± 1.2 |
| H- Cartilage C1 | 2.6 ± 0.1 | 47.6 | 83.9 ± 0.8 | 6.1 ± 0.8 | 47.9 ± 0.4 |
| H- Cartilage D1 | 2.5 ± 0.1 | 28.6 | 87.2 ± 0.3 | 9.6 ± 0.2 | 52.5 ± 0.8 |
| H- Cartilage E1 | 2.8 ± 0.2 | 22.2 | 83.0 ± 1.3 | 4.0 ± 0.1 | 45.4 ± 1.4 |
| JB-Muscle | 2.6 ± 0.1 | 2.3 | 88.8 ± 1.0 | 7.7 ± 0.7 | 33.0 ± 3.1 |
| JB-Ligament | 5.6 ± 0.3 | 9.2 | 69.9 ± 1.1 | 6.1 ± 0.3 | 59.1 ± 0.8 |
| JB-Tendon | 5.6 ± 0.3 | 2.9 | 80.4 ± 1.5 | 3.2 ± 0.2 | 68.8 ± 3.1 |
| JB-Synovium | 2.4 ± 0.2 | 4.4 | 85.5 ± 1.8 | 8.6 ± 0.7 | 0.0 ± 0.0 |
N/A indicates data not available.
a
The ratio of the MS ion abundance divided by the μg of DNA present per sample.
b
The percent of compositions terminating in a reducing end hexuronic acid.
c
The percent of under-sulfated Δ-unsaturated compositions per tissue sample.
d
The percent of unmodified linker region compositions as compared to all existing linker oligosaccharides.