Fig. 3.

Analysis of the promoter activity of block replacement mutants of the BmRPS12 promoter. (A) Promoter activity of 12–14 block replacements of the regions located 5′ and 3′ of the repeat domain. Grey bars indicate means and SDs of at least six independent transfections. The black separator indicates the position of the repeat domain. Asterisks indicate constructs whose activities were significantly lower than that seen in embryos transfected with the wild type promoter (P < 0.01). (B) Promoter activity of 3–4 nucleotide block replacements of the domains found to represent essential domains by the experiments presented in (A). The bars represent means and SDs of the promoter activity of each mutant. Each mutant was tested in at least six independent transfections. Similarly shaded bars represent constructs derived from each of the three essential domains identified in the experiments presented in (A). Asterisks indicate constructs whose activity was significantly lower than that seen in embryos transfected with the wild type promoter.