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. Author manuscript; available in PMC: 2008 Oct 31.
Published in final edited form as: J Pharmacol Exp Ther. 2008 Jun 3;326(3):871–878. doi: 10.1124/jpet.108.137919

Figure 1.

Figure 1

Detection of H3R and over-expression of β-ARK1(495−689) minigene in PC12-H3 cells. (A) Reverse-transcriptase PCR. cDNA was synthesized from total RNA prepared from PC12 and PC12-H3 cells and used as template in a PCR reaction. The PCR products were run on a 1.5 % agarose gel and detected under UV light. As controls, β-Actin primers were used in a parallel PCR reaction for amplification of a 353-bp segment. (B) Western blot analysis of β-ARK1(495−689) minigene. Cell lysates (20 μg) isolated from PC12-H3 and PC12-H3/β-ARK1 cells were resolved by SDS-PAGE and transferred to PVDF membrane. Blotting of the membrane with anti-β-ARK1 monoclonal antibody (1:500) revealed a ∼27 kDa specific band. Positions of standard molecular-mass markers of 26 and 37.4 kDa are shown on the left.