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. Author manuscript; available in PMC: 2008 Oct 31.
Published in final edited form as: J Pharmacol Exp Ther. 2008 Jun 3;326(3):871–878. doi: 10.1124/jpet.108.137919

Figure 2.

Figure 2

H3R activation reduces intracellular cAMP accumulation and dopamine exocytosis elicited by forskolin (10 μM) in NGF-differentiated rat pheocromocytoma cells stably transfected with human H3R (PC12-H3) and in PC12 cells transfected with both H3R and the Gβγ scavenger β-ARK1 (PC12-H3/β-ARK1). Panels A and B: intracellular cAMP accumulation (absolute values); panels C and D: dopamine release, in the absence or presence of the H3R agonist imetit (100 nM), either alone or in combination with the H3R antagonist clobenpropit (CBP, 50 nM), or after pre-treatment with PTX (200 ng/ml for 24 h). Dopamine release is expressed in percent increase above basal level. Bars are means ± SEM (n = 15−19 for A and B and n = 10−15 for C and D). Significantly different from control or forskolin (*p < 0.05 and **p < 0.01 by ANOVA followed by post hoc Dunnett's test).