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. Author manuscript; available in PMC: 2008 Oct 31.
Published in final edited form as: J Pharmacol Exp Ther. 2008 Jun 3;326(3):871–878. doi: 10.1124/jpet.108.137919

Figure 5.

Figure 5

Panel A: Calcium current traces and peak current density, recorded in NGF-differentiated PC12-H3 by stepping from the holding potential of −40 mV to the test potential of +10 mV, do not differ from calcium currents evoked in PC12-H3 cells transfected with the Gβγ scavenger β-ARK1 (PC12-H3/β-ARK1). Notably, H3R activation with imetit (100 nM) fails to reduce peak calcium current in PC12-H3/β-ARK1 cells. Bars represent means (± SEM; n = 20). Panel B: endogenous dopamine release was measured in PC12-H3/β-ARK1 cells in response to K+ (100 mM). Dopamine exocytosis was inhibited by nifedipine (5 μM), but not by imetit (100 nM), either alone or in combination with clobenpropit (CBP, 50 nM). Bars are means ± SEM of percent increases in dopamine release above control (n = 20−25). Significantly different from K+ alone (*p < 0.05 by ANOVA followed by post hoc Dunnett's test).