Figure 2.

Biofilm before and after inversion. The thin black dashed line shows the dominance of the bulk water signal if the T₁ inversion recovery pulse is not utilized. The spectra from a fresh biofilm after a T₁ inversion recovery pulse is shown with the bold black line and compared to the spectra from a sample of planktonic cells (dashed grey line). The bottom spectra are obtained with no diffusion encoding gradient pulses (q = 0 m⁻¹), while the upper spectra are obtained with the largest amplitude diffusion encoding gradient pulses (q = 2.85 × 10⁵ m⁻¹). Note that the inversion recovery pulse allows information to be obtained form all spectral peaks at all q values.