Table 2.
Statistical analysis of M2R subcellular distribution in VTA dendrites distinguished by DAT labeling and size
ASSOCIATION VARIABLES | DENDRITIC GROUP | X21 | p (Fisher) |
---|---|---|---|
Localization vs. Labeling | Small dendrites (<1μm) | 126.675 | <0.0001* |
Large dendrites (>1μm) | 40.728 | <0.0001* | |
Localization vs. Size | Single-labeled dendrites (M2R) | 70.913 | <0.0001* |
Dual-labeled dendrites (DAT+M2R) | 0.974 | 0.4064 | |
Labeling vs. Size | Cytoplasmic M2R localization | 3.314 | 0.0829 |
Plasmalemmal M2R localization | 0.693 | 0.4304 |
Chi-square values and statistical signification (p, Fisher's exact value) for association among subcellular localization (cytoplasmic vs. plasmalemmal), type of labeling (single M2R vs. dual DAT+M2R) and dendritic size (small vs. large) of M2R-immunogold labeling observed in different groups of dendrites of the rat ventral tegmental area (VTA) with regard to those variables. A total number of 1800 M2R-immunogold particles were assessed within 746 randomly sampled VTA dendrites in 4 animals.