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. 2008 Oct 22;5:125. doi: 10.1186/1743-422X-5-125

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Copyright © 2008 Tanaka et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Both of UL7 and ANT2 were exist in the mitochondrial fractions. Photograph of electrophoretically separated lysates of COS-7 cells infected with the indicated virus that transiently expressed (f)ANT2. The cytosolic fractions (lanes 1, 3, 5, and 7) and mitochondrial fractions (lanes 2, 4, 6, and 8), separated as described in Materials and Methods, were subjected to electrophoresis on a denaturing gel, transferred to a PVDF sheet, and reacted with the antibody to UL7 (first panel). The same membrane was reacted with the antibosy to the flag again (second panel), to the βactin (third panel) and to COX IV (bottom panel).