Figure 5.
TBPB regulates non-amyloidogenic APP processing. A, Western blots of conditioned media and cell lysates demonstrate increased production of APPsα and CTFα in cells treated with CCh and TBPB. B, Quantitation of APPsα band intensity shows a significant increase in APPsα shedding from CCh- and TBPB-treated cells (*p < 0.01, n = 7 across 3 separate experiments for TBPB; **p < 0.001, n = 7 across 3 separate experiments for CCh). C, Quantitation of CTFα band intensity demonstrates a significant increase in production of CTFα in CCh- and TBPB-treated cells (**p < 0.001, n = 16 across 6 separate experiments). D, ELISA measurements from conditioned media show a significant decrease in secreted Aβ40 from cells treated with either CCh or TBPB (**p < 0.001, n = 6 across 2 separate experiments for CCh; **p < 0.001, n = 9 across 3 separate experiments for TBPB).