Table 4.
Mean sequence coverage and counts of identified CID spectra
| Mean % sequence coverage (mean spectrum count)
| ||||||||
|---|---|---|---|---|---|---|---|---|
| Protein | Mix 1 | Mix 2 | Mix 3 | Mix 4 | ||||
| Actin, aortic smooth muscle | 44.2 | (48.0) | 48.9 | (54.5) | 38.5 | (51.8) | 30.9 | (28.5) |
| Alkaline phosphotase | 86.2 | (218.7) | 82.8 | (161.8) | 91.7 | (150.0) | 83.5 | (95.8) |
| Alpha-amylase | 56.6 | (68.4) | 63.4 | (58.1) | 42.0 | (51.2) | 35.2 | (24.6) |
| Alpha-lactalbumin | 73.9 | (32.7) | 67.9 | (37.8) | 50.5 | (14.2) | 43.6 | (13.9) |
| Beta-casein | 37.1 | (28.0) | 66.8 | (51.0) | 30.4 | (15.5) | 22.9 | (5.9) |
| Beta-galactosidase | 79.1 | (292.4) | 87.4 | (273.7) | 51.8 | (126.9) | 46.0 | (78.4) |
| Beta-lactoglobulin | 77.4 | (54.4) | 80.8 | (47.7) | 55.9 | (36.1) | 53.8 | (21.5) |
| Carbonic anhydrase 2 | 78.2 | (69.6) | 83.2 | (85.3) | 74.5 | (76.0) | 53.9 | (27.7) |
| Catalase | 72.6 | (108.7) | 75.4 | (99.9) | 57.7 | (75.2) | 53.1 | (57.8) |
| Cytochrome c | 67.3 | (40.8) | 66.0 | (32.6) | 69.6 | (24.6) | 60.5 | (14.4) |
| Glyceraldehyde-3-phosphate dehydrogenase | 73.2 | (261.2) | 78.3 | (173.7) | 69.6 | (126.7) | 59.2 | (62.7) |
| Glycogen phosphorylase, muscle form | 71.5 | (230.2) | 76.5 | (180.0) | 54.0 | (115.3) | 52.1 | (82.1) |
| Mannose-6-phosphate isomerase | 80.4 | (88.2) | 91.5 | (122.6) | 42.9 | (34.0) | 38.1 | (20.2) |
| Myoglobin | 74.6 | (35.8) | 79.4 | (43.0) | 48.2 | (12.6) | 46.0 | (9.4) |
| Myosin light chain 1, skeletal muscle isoform | 87.8 | (61.5) | 85.1 | (55.8) | 44.3 | (19.1) | 43.3 | (16.3) |
| Ovalbumin | 70.6 | (92.8) | 80.4 | (112.5) | 31.2 | (37.8) | 23.1 | (17.7) |
| Serotransferrin | 74.0 | (167.8) | 70.4 | (139.5) | 67.6 | (170.9) | 61.6 | (102.3) |
| Serum albumin | 72.8 | (155.0) | 74.0 | (145.8) | 64.2 | (75.0) | 65.3 | (83.9) |
|
| ||||||||
| Average coverage (total mean spectra count) | 71.5 | (2054.2) | 75.5 | (1875.3) | 54.7 | (1212.9) | 48.5 | (763.1) |