Table 3.

PCR amplification conditions used in the present study.

PCR No	Initial denaturation	Denaturation	Annealing	Extension	No of cycles	Final extension
1	95°C; 15 min	95°C; 30 s	56°C; 30 s	72°C; 1 min	30	72°C; 10 min
2	95°C; 15 min	95°C; 30 s	62°C; 30 s	72°C; 1 min	30	72°C; 10 min
3	95°C; 15 min	95°C; 30 s	55°C; 30 s	72°C; 1 min	30	72°C; 10 min
4	95°C; 15 min	95°C; 30 s	57°C; 30 s	72°C; 1 min	37	72°C; 10 min
5	95°C; 15 min	95°C; 30 s	65°C, 30 s	72°C; 30 s	5	72°C; 10 min
		95°C; 30 s	60°C; 30 s	72°C; 30 s	25
		95°C; 30 s	55°C; 30 s	72°C; 30 s	5