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. Author manuscript; available in PMC: 2008 Nov 3.
Published in final edited form as: Immunol Rev. 2005 Jun;205:220–228. doi: 10.1111/j.0105-2896.2005.00255.x

Fig. 5. CD154 expression and NFκB binding of aged CD4+ T cells can be enhanced by culturing with exogenous interleukin (IL)-2.

Fig. 5

Purified populations of transgenic (Tg) CD4+ T cells from young (black) and aged (red) mice were stimulated with peptide antigen and antigen-presenting cell (DCEK—intercellular adhesion molecule fibroblasts) with or without exogenous IL-2 (80 U/ml). (A) At the indicated time points, the expression of CD154 was determined on the CD4+Vβ3+ populations by flow cytometry. (B) After 4 days of culture, equal numbers of each effector population were stimulated with anti-Vβ3/anti-CD28 for 4 h and nuclear extracts were then prepared. Electrophoretic mobility shift assays were performed using [32P]dCTP-labeled probe for NFκB.