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. Author manuscript; available in PMC: 2008 Nov 3.
Published in final edited form as: Immunol Rev. 2005 Jun;205:220–228. doi: 10.1111/j.0105-2896.2005.00255.x

Fig. 6. Young donor CD4+ T cells exhibit good cognate function in young and aged hosts.

Fig. 6

Purified populations of transgenic (Tg) CD4+ T cells from young mice were transferred i.v. to young (black) or aged (red) CD4KO hosts (106 per host). Hosts were immunized with 200 μg of 4-hydroxy-3-nitrophenyl acetyl conjugated to PCC (NP-PCC)/alum i.p. on the same day. (A) On day 14, the number of NP-specific host B cells was determined by staining with NP-APC. The number of NP+ germinal center (GC) phenotype B cells (CD38lo PNAhi) was also determined by flow cytometry. (B) On day 14, serum was collected from each host and assayed for the presence of NP-specific immunoglobulin G1 (IgG1) antibodies.