Fig. 6.

Comparison of MS-identified phosphosites on BKα with sites predicted by computer algorithms. A, phosphorylation site predictions for all Ser/Thr residues of BKα (including splice variations) by the indicated algorithms. Scores (given as relative values) considered significant by the individual algorithms are marked by the gray bar and by non-shaded symbols (for predictions by Scansite). Phosphorylation sites predicted by PhosphoSite are given as filled green circles; MS-identified sites are denoted by black (unambiguous sites) and gray rectangles (ambiguous sites). Ser/Thr residues inaccessible to our MS analyses are marked by horizontal dashes. B, overlap of phosphosites as predicted by at least three or four computer algorithms (left and middle panel; for details on the 7 algorithms used see Supplemental Material) or by phosphosite with the 30 sites identified by the MS analyses presented here. Predicted sites are depicted as blue circles, identified sites by red circles.