Fig. 3.

Composite of Western analyses confirming phosphorylation-dependent binding of selected human proteins. Human brain proteins bind the carboxyl terminus of α-synuclein in a sequence- and phosphorylation-dependent manner. A composite of Western blots of total human synaptosomal protein pulled down by magnetic beads is shown. Each lane is labeled with the peptide bound to the magnetic beads that was used for each pulldown (scrambled, NP, and pS129). Pulldown experiments were performed as in Fig. 1. Eluted proteins bound to each peptide were separated by SDS-PAGE (4–20%), and Western analysis was performed with various antibodies as indicated to the right of the Western blots as described under “Experimental Procedures.” HC, heavy chain.