Fig. 4.

Competition with the NP peptide pulldown by full-length recombinant human α-synuclein. Pulldown experiments using biotinylated NP peptide were performed in the presence or absence of a 3× or 10× molar excess of recombinant full-length human α-synuclein (residues 1–140) or a control protein consisting of truncated α-synuclein lacking the terminal 16 amino acids (residues 1–124). Eluted proteins bound to the biotinylated peptide were separated by SDS-PAGE (4–20%), and Western analysis was performed using specific antibodies. The eluted protein mixture was stained with GelCode Blue to identify the streptavidin, which served as a gel loading control.