Table II.
Proteins with preferential affinity for the Ser-129 phosphorylated α-synuclein interaction network
To be considered specific a protein had to have peptide hits greater than 3 times that of the control. Comparisons are shown only for proteins identified by 10 or more peptide (Pep) hits. A protein was considered to show preferential enrichment if it had 2 times the number of peptide hits in the pS129 pulldown relative to the NP peptide. Ion current -fold changes are also shown as confirmation of peptide hit quantification. n/a, not applicable; Snare, soluble N-ethylmaleimide-sensitive factor attachment protein receptors; CNPase, 2′,3′-cyclic-nucleotide 3′-phosphodiesterase.
| UniProtKB/Swiss-Prot entry name | Primary accession number | Gene name | Pep hits
|
pS129 affinity
|
Unique peptides
|
Description | |||
|---|---|---|---|---|---|---|---|---|---|
| pS129 | NP | Normalized Pep hits | Normalized ion current | pS129 | NP | ||||
| Signaling | |||||||||
| Q6PJ87_MOUSE | Q6PJ87 | Csnk1a1 | 24 | 0 | n/a | n/a | 7 | 0 | Casein kinase 1, α1 |
| MARK1_MOUSE | Q8VHJ5 | Mark1 | 13 | 0 | n/a | n/a | 4 | 0 | Serine/threonine-protein kinase MARK1 |
| Q5RJI5_MOUSE | Q5RJI5 | Brsk1 | 11 | 0 | n/a | n/a | 2 | 0 | BR serine/threonine kinase 1 |
| KCC2B_MOUSE | P28652 | Camk2b | 185 | 6 | 25.2 | 36.7 | 7 | 3 | CaM kinase II β chain |
| Q6PHZ2_MOUSE | Q6PHZ2 | Camk2d | 79 | 3 | 21.5 | 29.8 | 4 | 1 | CaM kinase II δ chain |
| Q80TN1_MOUSE | Q80TN1 | Camk2a | 168 | 13 | 10.6 | 25.8 | 7 | 4 | MKIAA0968 protein (fragment) |
| MARK2_MOUSE | Q05512 | Mark2 | 46 | 4 | 9.4 | 10 | 1 | Serine/threonine-protein kinase MARK2 | |
| PP1A_MOUSE | P62137 | Ppp1ca | 16 | 2 | 6.5 | 8.5 | 3 | 2 | Serine/threonine-protein phosphatase PP1-α catalytic |
| Cytoskeleton | |||||||||
| Q8C8R3_MOUSE | Q8C8R3 | Ank2 | 73 | 0 | n/a | n/a | 13 | 0 | Similar to Ankyrin-2 |
| Q6PCN2_MOUSE | Q6PCN2 | Ank2 | 48 | 0 | n/a | n/a | 11 | 0 | Ankyrin 2, brain |
| AINX_MOUSE | P46660 | Ina | 40 | 0 | n/a | n/a | 9 | 0 | α-Internexin |
| MYO5A_MOUSE | Q99104 | Myo5a | 28 | 0 | n/a | n/a | 6 | 0 | Myosin-5A |
| Q3UMG4_MOUSE | Q3UMG4 | Ina | 22 | 0 | n/a | n/a | 5 | 0 | Internexin neuronal intermediate filament protein |
| Q3THE2_MOUSE | Q3THE2 | Mylc2b | 22 | 0 | n/a | n/a | 5 | 0 | Myosin regulatory light chain |
| BCAS1_MOUSE | Q80YN3 | Bcas1 | 14 | 0 | n/a | n/a | 1 | 0 | Novel amplified in breast cancer 1 homolog |
| CTNB1_MOUSE | Q02248 | Ctnnb1 | 13 | 0 | n/a | n/a | 6 | 0 | β-Catenin |
| DYL1_MOUSE | P63168 | Dynll1 | 12 | 0 | n/a | n/a | 1 | 0 | Dynein light chain 1 |
| Q3TY37_MOUSE | Q3TY37 | Ctnna2 | 10 | 0 | n/a | n/a | 5 | 0 | Catenin α2 |
| SPTA2_MOUSE | P16546 | Spna2 | 256 | 4 | 52.3 | 298.1 | 33 | 3 | Spectrin α chain, brain |
| MYH10_MOUSE | Q61879 | Myh10 | 48 | 1 | 39.2 | 398.8 | 14 | 1 | Myosin-10 (myosin heavy chain, non-muscle IIb) |
| Q3V1V5_MOUSE | Q3V1V5 | Spna2 | 143 | 3 | 38.9 | 98.8 | 18 | 2 | Spectrin α chain, brain |
| NFL_MOUSE | P08551 | Nefl | 37 | 1 | 30.2 | 217.8 | 9 | 1 | Neurofilament triplet L protein |
| TRIM3_MOUSE | Q9R1R2 | Trim3 | 33 | 1 | 26.9 | 134.4 | 7 | 1 | Tripartite motif-containing protein 3 |
| CAZA2_MOUSE | P47754 | Capza2 | 34 | 2 | 13.9 | 112.3 | 6 | 1 | F-actin capping protein α-2 subunit |
| DCTN2_MOUSE | Q99KJ8 | Dctn2 | 16 | 1 | 13.1 | 47.8 | 5 | 1 | Dynactin subunit 2 |
| MLP3A_MOUSE | Q91VR7 | Map1lc3a | 16 | 1 | 13.1 | 10.8 | 2 | 1 | Microtubule-associated proteins 1A/1B light chain 3A |
| SPTB2_MOUSE | Q62261 | Spnb2 | 341 | 22 | 12.7 | 64.4 | 55 | 15 | Spectrin β chain, brain 1 |
| Q80UE4_MOUSE | Q80UE4 | Epb41l2 | 26 | 2 | 10.6 | 36.7 | 7 | 2 | Protein 4.1G |
| TMOD2_MOUSE | Q9JKK7 | Tmod2 | 11 | 1 | 9.0 | 40.8 | 5 | 1 | Tropomodulin-2 |
| E41L3_MOUSE | Q9WV92 | Epb41l3 | 75 | 8 | 7.7 | 17.3 | 11 | 5 | Band 4.1-like protein 3 |
| ACTB_MOUSE | P60710 | Actb | 182 | 21 | 7.1 | 31.1 | 15 | 5 | Actin, cytoplasmic 1 (β-actin) |
| Q9QZ83_MOUSE | Q9QZ83 | Actg1 | 173 | 21 | 6.7 | 30.5 | 13 | 5 | γ-Actin-like protein |
| Q7TSJ2_MOUSE | Q7TSJ2 | Mtap6 | 62 | 10 | 5.1 | 10.7 | 4 | 2 | Microtubule-associated protein 6 |
| MAP1B_MOUSE | P14873 | Mtap1b | 992 | 165 | 4.9 | 9.2 | 45 | 25 | Microtubule-associated protein 1B |
| TBB6_MOUSE | Q922F4 | Tubb6 | 51 | 9 | 4.6 | 15.3 | 5 | 4 | Tubulin β-6 chain |
| DYL2_MOUSE | Q9D0M5 | Dynll2 | 22 | 4 | 4.5 | 32.3 | 2 | 1 | Dynein light chain 2 |
| P25A_MOUSE | Q7TQD2 | Tppp | 11 | 2 | 4.5 | 8.6 | 2 | 1 | Tubulin polymerization-promoting protein (TPPP) |
| Q80Y54_MOUSE | Q80Y54 | Tubb4 | 100 | 29 | 2.8 | 15.3 | 12 | 10 | Tubulin β-4 |
| TBB5_MOUSE | P99024 | Tubb5 | 123 | 36 | 2.8 | 14.9 | 12 | 10 | Tubulin β-5 chain |
| TBB3_MOUSE | Q9ERD7 | Tubb3 | 99 | 29 | 2.8 | 11.4 | 8 | 7 | Tubulin β-3 chain |
| TBA4_MOUSE | P68368 | Tuba4 | 131 | 43 | 2.5 | 11.6 | 9 | 7 | Tubulin α-4 chain |
| ACTY_MOUSE | Q8R5C5 | Actr1b | 33 | 11 | 2.4 | 2.5 | 6 | 3 | β-Centractin |
| ACTZ_MOUSE | P61164 | Actr1a | 30 | 10 | 2.4 | 2.2 | 6 | 2 | α-Centractin |
| Endocytosis | |||||||||
| AP1B1_MOUSE | O35643 | Ap1b1 | 26 | 0 | n/a | n/a | 6 | 0 | AP-1 complex subunit β-1 |
| AP2M1_MOUSE | P84091 | Ap2m1 | 26 | 1 | 21.2 | 48.2 | 5 | 1 | AP-2 complex subunit μ-1 |
| AP2B1_MOUSE | Q9DBG3 | Ap2b1 | 82 | 8 | 8.4 | 20.3 | 15 | 4 | AP-2 complex subunit β-1 |
| AP2A1_MOUSE | P17426 | Ap2a1 | 89 | 15 | 4.8 | 25.5 | 14 | 4 | AP-2 complex subunit α-1 |
| CLH_MOUSE | Q68FD5 | Cltc | 105 | 25 | 3.4 | 8.5 | 26 | 13 | Clathrin heavy chain |
| 14-3-3 chaperones | |||||||||
| 1433E_MOUSE | P62259 | Ywhae | 24 | 0 | n/a | n/a | 4 | 0 | 14-3-3 protein ε |
| 1433Z_MOUSE | P63101 | Ywhaz | 26 | 1 | 21.2 | 103.8 | 5 | 1 | 14-3-3 protein ζ/δ |
| 1433G_MOUSE | P61982 | Ywhag | 23 | 2 | 9.4 | 28.5 | 4 | 1 | 14-3-3 protein γ |
| Neural adhesion molecule | |||||||||
| NTRI_MOUSE | Q99PJ0 | Hnt | 33 | 2 | 13.5 | 185.3 | 4 | 1 | Neurotrimin precursor |
| CNTN1_MOUSE | P12960 | Cntn1 | 78 | 16 | 4.0 | 13.2 | 18 | 7 | Contactin-1 precursor |
| Glutamate transport membrane | |||||||||
| EAA1_MOUSE | P56564 | Slc1a3 | 132 | 38 | 2.8 | 4.0 | 3 | 2 | Excitatory amino acid transporter 1 |
| Snare complex | |||||||||
| SNIP_MOUSE | Q9QWI6 | P140 | 15 | 5 | 2.4 | 17.4 | 4 | 2 | SNAP-25-interacting protein |
| Mitochondrial proteins | |||||||||
| Q8JZU2_MOUSE | Q8JZU2 | Slc25a1 | 13 | 2 | 5.3 | 4.6 | 3 | 1 | Solute carrier family 25 |
| GHC1_MOUSE | Q9D6M3 | Slc25a22 | 10 | 4 | 2.0 | 5.8 | 5 | 4 | Solute carrier family 25 member 22 |
| LACTB_MOUSE | Q9EP89 | Lactb | 72 | 0 | n/a | n/a | 9 | 0 | Serine β-lactamase-like protein LACTB |
| TFAM_MOUSE | P40630 | Tfam | 15 | 0 | n/a | n/a | 3 | 0 | Transcription factor A, mitochondrial precursor |
| ATPase | |||||||||
| VA0D_MOUSE | P51863 | Atp6v0d1 | 29 | 6 | 3.9 | 2.6 | 6 | 3 | Vacuolar ATP synthase subunit d |
| Q8CHX2_MOUSE | Q8CHX2 | Atp6v1a | 53 | 13 | 3.3 | 3.7 | 10 | 6 | Vacuolar ATP synthase catalytic subunit A |
| G proteins | |||||||||
| GBB1_MOUSE | P62874 | Gnb1 | 67 | 21 | 2.6 | 3.8 | 6 | 5 | Transducin β chain 1 |
| GBB2_MOUSE | P62880 | Gnb2 | 43 | 16 | 2.2 | 3.4 | 6 | 4 | Transducin β chain 2 |
| Ribosomal proteins | |||||||||
| RS7_MOUSE | P62082 | Rps7 | 69 | 26 | 2.2 | 6.6 | 8 | 6 | 40 S ribosomal protein S7 |
| RS10_MOUSE | P63325 | Rps10 | 11 | 4 | 2.2 | 2.2 | 2 | 2 | 40 S ribosomal protein S10 |
| Miscellaneous | |||||||||
| LSAMP_MOUSE | Q8BLK3 | Lsamp | 20 | 0 | n/a | n/a | 5 | 0 | Limbic system-associated membrane protein precursor |
| CALM_MOUSE | P62204 | Calm3 | 16 | 0 | n/a | n/a | 3 | 0 | CaM |
| Q6DFY2_MOUSE | Q6DFY2 | Opcml | 14 | 0 | n/a | n/a | 3 | 0 | Opioid-binding protein/cell adhesion molecule-like |
| ROA2_MOUSE | O88569 | Hnrpa2b1 | 10 | 0 | n/a | n/a | 1 | 0 | Heterogeneous nuclear ribonucleoproteins A2/B1 |
| H2A2B_MOUSE | Q64522 | Hist2h2ab | 11 | 1 | 9.0 | 40.0 | 3 | 1 | Histone H2A type 2-B |
| CN37_MOUSE | P16330 | Cnp1 | 74 | 7 | 8.6 | 12.3 | 7 | 4 | CNPase |
| CSKI1_MOUSE | Q6P9K8 | Caskin1 | 49 | 5 | 8.0 | 13.6 | 7 | 2 | CASK-interacting protein 1 |
| CENG1_MOUSE | Q3UHD9 | Centg1 | 155 | 17 | 7.4 | 21.6 | 14 | 5 | Centaurin-γ 1 |
| USMG5_MOUSE | Q78IK2 | Usmg5 | 10 | 2 | 4.1 | 21.4 | 2 | 1 | Up-regulated during skeletal muscle growth protein 5 |
| THY1_MOUSE | P01831 | Thy1 | 58 | 14 | 3.4 | 7.8 | 5 | 2 | Thy-1 membrane glycoprotein precursor |
| ALDOA_MOUSE | P05064 | Aldoa | 117 | 37 | 2.6 | 4.8 | 8 | 7 | Fructose-bisphosphate aldolase A |
| SYPH_MOUSE | Q62277 | Syp | 11 | 4 | 2.2 | 5.6 | 2 | 1 | Synaptophysin |