Table IV.
Proteins with preferential affinity for the non-phosphorylated α-synuclein interaction network
Criteria were the same as in Table II. A protein was considered to show preferential affinity if it had 2 times the number of peptide (Pep) hits in the non-phosphorylated pulldown relative to the phosphorylated. n/a, not applicable.
| UniProtKB/Swiss-Prot entry name | Primary accession number | Gene name | Pep hits
|
NP affinity
|
Unique peptides
|
Description | |||
|---|---|---|---|---|---|---|---|---|---|
| NP | pS129 | Normalized Pep hits | Normalized ion current | NP | pS129 | ||||
| Complex I | |||||||||
| NDUAB_MOUSE | Q9D8B4 | Ndufa11 | 28 | 0 | n/a | n/a | 3 | 0 | NADH-ubiquinone oxidoreductase subunit B14.7 |
| NU1M_MOUSE | P03888 | ND1 | 22 | 0 | n/a | n/a | 1 | 0 | NADH-ubiquinone oxidoreductase chain 1 |
| NIPM_MOUSE | Q99LY9 | Ndufs5 | 14 | 0 | n/a | n/a | 2 | 0 | NADH-ubiquinone oxidoreductase 15-kDa subunit |
| NU5M_MOUSE | P03921 | ND5 | 47 | 2 | 28.8 | 22.9 | 3 | 2 | NADH-ubiquinone oxidoreductase chain 5 |
| NUBM_MOUSE | Q91YT0 | Ndufv1 | 164 | 14 | 14.3 | 28.1 | 11 | 5 | NADH-ubiquinone oxidoreductase 51-kDa subunit |
| NDUBB_MOUSE | O09111 | Ndufb11 | 35 | 3 | 14.3 | 12.1 | 5 | 2 | NADH-ubiquinone oxidoreductase ESSS subunit |
| NUYM_MOUSE | Q9CXZ1 | Ndufs4 | 11 | 1 | 13.5 | 10.0 | 3 | 1 | NADH-ubiquinone oxidoreductase 18-kDa subunit |
| NDUB3_MOUSE | Q9CQZ6 | Ndufb3 | 20 | 2 | 12.2 | 4.9 | 2 | 1 | NADH-ubiquinone oxidoreductase B12 subunit |
| NUCM_MOUSE | Q91WD5 | Ndufs2 | 167 | 26 | 7.9 | 14.3 | 14 | 8 | NADH-ubiquinone oxidoreductase 49-kDa subunit |
| NDUB8_MOUSE | Q9D6J5 | Ndufb8 | 12 | 2 | 7.3 | 6.9 | 3 | 1 | NADH-ubiquinone oxidoreductase ASHI subunit |
| NUIM_MOUSE | Q8K3J1 | Ndufs8 | 71 | 12 | 7.2 | 23.8 | 6 | 3 | NADH-ubiquinone oxidoreductase 23-kDa subunit |
| NDUB6_MOUSE | Q3UIU2 | Ndufb6 | 23 | 4 | 7.0 | 8.3 | 2 | 2 | NADH-ubiquinone oxidoreductase B17 subunit |
| NDUB7_MOUSE | Q9CR61 | Ndufb7 | 97 | 17 | 7.0 | 11.5 | 9 | 4 | NADH-ubiquinone oxidoreductase B18 subunit |
| NDUAA_MOUSE | Q99LC3 | Ndufa10 | 74 | 13 | 7.0 | 18.9 | 12 | 6 | NADH-ubiquinone oxidoreductase 42-kDa subunit |
| NUAM_MOUSE | Q91VD9 | Ndufs1 | 491 | 89 | 6.8 | 17.1 | 28 | 15 | NADH-ubiquinone oxidoreductase 75-kDa subunit |
| NUKM_MOUSE | Q9DC70 | Ndufs7 | 69 | 13 | 6.5 | 33.9 | 6 | 3 | NADH-ubiquinone oxidoreductase 20-kDa subunit |
| NU2M_MOUSE | P03893 | Mtnd2 | 15 | 3 | 6.1 | 8.0 | 2 | 1 | NADH-ubiquinone oxidoreductase chain 2 |
| N4BM_MOUSE | Q9CQ54 | Ndufc2 | 18 | 4 | 5.5 | 15.0 | 2 | 1 | NADH-ubiquinone oxidoreductase subunit B14.5b |
| NUHM_MOUSE | Q9D6J6 | Ndufv2 | 95 | 22 | 5.3 | 13.3 | 9 | 7 | NADH-ubiquinone oxidoreductase 24-kDa subunit |
| NDUB9_MOUSE | Q9CQJ8 | Ndufb9 | 73 | 17 | 5.3 | 13.5 | 3 | 2 | NADH-ubiquinone oxidoreductase B22 subunit |
| NDUA6_MOUSE | Q9CQZ5 | Ndufa6 | 29 | 7 | 5.1 | 15.2 | 3 | 1 | NADH-ubiquinone oxidoreductase B14 subunit |
| NDUB5_MOUSE | Q9CQH3 | Ndufb5 | 21 | 6 | 4.3 | 15.0 | 3 | 2 | NADH-ubiquinone oxidoreductase SGDH subunit |
| Q6GTD3_MOUSE | Q6GTD3 | Ndufa9 | 87 | 25 | 4.3 | 7.5 | 9 | 6 | NADH dehydrogenase (ubiquinone) 1α subcomplex, 9 |
| NU4M_MOUSE | P03911 | ND4 | 24 | 7 | 4.2 | 7.8 | 4 | 3 | NADH-ubiquinone oxidoreductase chain 4 |
| NDUBA_MOUSE | Q9DCS9 | Ndufb10 | 131 | 39 | 4.1 | 11.7 | 7 | 6 | NADH-ubiquinone oxidoreductase PDSW subunit |
| NDUA5_MOUSE | Q9CPP6 | Ndufa5 | 17 | 6 | 3.5 | 5.4 | 3 | 2 | NADH-ubiquinone oxidoreductase 13-kDa B subunit |
| NDUAC_MOUSE | Q7TMF3 | Ndufa12 | 16 | 6 | 3.3 | 10.0 | 4 | 2 | NADH-ubiquinone oxidoreductase subunit B17.2 |
| NUGM_MOUSE | Q9DCT2 | Ndufs3 | 113 | 43 | 3.2 | 10.7 | 10 | 7 | NADH-ubiquinone oxidoreductase 30-kDa subunit |
| NDUAD_MOUSE | Q9ERS2 | Ndufa13 | 65 | 26 | 3.1 | 13.8 | 5 | 5 | NADH-ubiquinone oxidoreductase B16.6 subunit |
| NDUA8_MOUSE | Q9DCJ5 | Ndufa8 | 40 | 18 | 2.7 | 10.8 | 5 | 3 | NADH-ubiquinone oxidoreductase 19-kDa subunit |
| NDUB4_MOUSE | Q9CQC7 | Ndufb4 | 21 | 11 | 2.3 | 9.1 | 2 | 2 | NADH-ubiquinone oxidoreductase B15 subunit |
| Complex III | |||||||||
| UCR6_MOUSE | Q9D855 | Uqcrb | 18 | 2 | 11.0 | 30.6 | 3 | 1 | Ubiquinol-cytochrome c reductase complex 14-kDa protein |
| UQCR1_MOUSE | Q9CZ13 | Uqcrc1 | 289 | 62 | 5.7 | 14.5 | 14 | 9 | Ubiquinol-cytochrome c reductase complex core protein I |
| CY1_MOUSE | Q9D0M3 | Cyc1 | 168 | 40 | 5.1 | 14.5 | 7 | 5 | Cytochrome c1, heme protein, mitochondrial precursor |
| UCRI_MOUSE | Q9CR68 | Uqcrfs1 | 118 | 36 | 4.0 | 9.3 | 8 | 5 | Ubiquinol-cytochrome c reductase iron-sulfur subunit |
| UQCR2_MOUSE | Q9DB77 | Uqcrc2 | 215 | 66 | 4.0 | 9.4 | 15 | 13 | Ubiquinol-cytochrome c reductase complex core protein 2 |
| UCR10_MOUSE | Q8R1I1 | Uqcr10 | 19 | 7 | 3.3 | 3.6 | 2 | 1 | Ubiquinol-cytochrome c reductase complex 7.2-kDa protein |
| UCRQ_MOUSE | Q9CQ69 | Uqcrq | 16 | 7 | 2.8 | 8.4 | 1 | 1 | Ubiquinol-cytochrome c reductase complex 9.5-kDa protein |
| Complex IV | |||||||||
| COX6C_MOUSE | Q9CPQ1 | Cox6c | 20 | 2 | 12.2 | 44.6 | 2 | 1 | Cytochrome c oxidase polypeptide Vic |
| Q9MD68_MOUSE | Q9MD68 | mt-Co1 | 19 | 3 | 7.8 | 8.0 | 1 | 1 | Cytochrome oxidase subunit 1 |
| COX7R_MOUSE | Q61387 | Cox7a2l | 15 | 3 | 6.1 | 7.3 | 2 | 1 | Cytochrome c oxidase subunit VIIa-related protein |
| CX7A2_MOUSE | P48771 | Cox7a2 | 57 | 12 | 5.8 | 13.1 | 2 | 2 | Cytochrome c oxidase subunit VIIa-L |
| COX5B_MOUSE | P19536 | Cox5b | 15 | 4 | 4.6 | 12.5 | 1 | 1 | Cytochrome c oxidase polypeptide Vb |
| COX2_MOUSE | P00405 | COX2 | 192 | 73 | 3.2 | 9.3 | 4 | 4 | Cytochrome c oxidase subunit 2 |
| COX5A_MOUSE | P12787 | Cox5a | 28 | 13 | 2.6 | 2.8 | 3 | 2 | Cytochrome c oxidase polypeptide Va |
| CX6A1_MOUSE | P43024 | Cox6a1 | 21 | 11 | 2.3 | 1.6 | 1 | 1 | Cytochrome c oxidase polypeptide Via |
| Mitochondrial membrane proteins | |||||||||
| Q3U5Y8_MOUSE | Q3U5Y8 | Fam82c | 18 | 0 | n/a | n/a | 4 | 0 | Protein FAM82C |
| GLPK_MOUSE | Q64516 | Gyk | 10 | 2 | 6.1 | 4.0 | 2 | 1 | Glycerol kinase |
| Synaptic vesicle proteins | |||||||||
| VAPA_MOUSE | Q9WV55 | Vapa | 81 | 17 | 5.8 | 21.2 | 6 | 3 | Vesicle-associated membrane protein-associated protein A |
| VAPB_MOUSE | Q9QY76 | Vapb | 49 | 11 | 5.5 | 13.5 | 3 | 3 | Vesicle-associated membrane protein-associated protein B |
| Mitochondrial transport membrane proteins | |||||||||
| Q3TWD3_MOUSE | Q3TWD3 | Samm50 | 25 | 8 | 3.8 | 3.0 | 10 | 6 | SAM50-like protein CGI-51 homolog |
| Q3TBZ2_MOUSE | Q3TBZ2 | Tomm40 | 16 | 8 | 2.4 | 1.2 | 4 | 3 | Translocase of outer mitochondrial membrane 40 homolog |
| Miscellaneous | |||||||||
| STXB1_MOUSE | O08599 | Stxbp1 | 111 | 50 | 2.7 | 5.3 | 17 | 14 | Syntaxin-binding protein 1 (Unc-18-1) |