Fig. 1. The domain structure of ADAMTS13 and various carboxyl-terminal truncated mutants.
Full-length ADAMTS13 (FL) consists of a metalloprotease domain (M), a disintegrin domain (D), the first TSP1 repeat (T), a Cys-rich domain (C), and a spacer domain (S) followed by seven more TSP1 repeats (T2–8) and two CUB domains. The DTCS domains are located in the proximal C terminus of AD-AMTS13. The TSP1 2–8 and two CUB domains are located in the middle and the distal C termini of ADAMTS13, respectively. Each construct is tagged at its C terminus by a V5-His epitope derived from pcDNA3.1 V5-His TOPO.