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. Author manuscript; available in PMC: 2008 Nov 3.
Published in final edited form as: J Biol Chem. 2005 Jun 23;280(33):29428–29434. doi: 10.1074/jbc.M505513200

Fig. 4. Relative proteolytic activity of ADAMTS13 and the carboxyl-terminal truncated mutants.

Fig. 4

A, Western blot with anti-GST detects cleavage of GST-VWF73-H (200 ng) by 14 nM full-length AD-AMTS13 (FL) and the various carboxyl-terminal deleted mutants for 2 h. Lanes 1–7 correspond to the constructs negative control (Con), M, MD, MDT, MDTC, MDTCS, and delCUB, respectively, as illustrated in Fig. 1. The arrow indicates the intact substrate, and the arrowhead indicates the cleaved product. B, the signal on the luminogram was quantified by densitometry with ImageJ and is shown as the means ± S.D. from three independent experiments. Sequential addition of the proximal carboxyl-terminal domains gradually increases the proteolytic activity.

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