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. Author manuscript; available in PMC: 2008 Nov 3.
Published in final edited form as: J Biol Chem. 2005 Jun 23;280(33):29428–29434. doi: 10.1074/jbc.M505513200

Fig. 6. Binding of ADAMTS13 domains to GST-VWF73-H in solution.

Fig. 6

Purified disintegrin domain (Dis) (A), first TSP1 (TSP1) (B), Cys-rich domain (CysR) (C), and spacer domain (Spa) (D) bound to GST-VWF73-H (VWF73) in solution were pulled down by glutathione-Sepharose 4B and detected by Western blotting with anti-V5 IgG and chemiluminescent reagents. IN, amount of initial ADAMTS13 domain used for the assay. No ADAMTS13 domain was pulled down in the reactions omitting either GST-VWF73-H (−) or the V5-His-tagged AD-AMTS13 domain (−).

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