Table II. Binding of ADAMTS13 and various mutants or the proximal C-terminal domains to GST-VWF73-H.
Binding of full-length ADAMTS13 (FL) and the mutants (MDTCS and DTCS) or individual domains (M, Dis, TSP1, CysR, and Spa) to immobilized GST-VWF73-H was determined on an enzyme-linked immunosorbent assay plate. Kd values were calculated as described under “Experimental Procedures.” ND, Kd for this construct was not determined. Entries represent the average ± S.E. (n = 3).
| Constructs | Kd |
|---|---|
| nm | |
| FL | 4.3 ± 1.3 |
| MDTCS | 7.0 ± 1.9 |
| DTCS | 13 ± 2.1 |
| M | ND |
| Dis | 489 ± 42 |
| TSP1 | 136 ± 4.0 |
| CysR | 121 ± 5.0 |
| Spa | 108 ± 14 |