Figure 7.

β-Amylolysis experiments carried out on purified amylopectin from isa1-1 isa3-1 and isa3-1 pu1-1 double mutants. After purification, amylopectin was submitted to degradation by β-amylase and subsequent enzymatic debranching. Linear chains were then subjected to HPAEC-PAD. A, Differential plots before and after β-amylolysis (i.e. profile after β-amylolysis minus profile before β-amylolysis) for amylopectin of wild-type, isa1-1 isa3-1, and isa3-1 pu1-1 lines. B, Differential plots between mutant and wild-type amylopectin after β-amylolysis (i.e. profile of mutant amylopectin after β-amylolysis minus profile of wild-type amylopectin after β-amylolysis).