Figure 4.

SNP-elicited ethylene production in tobacco leaves. A, Ethylene production was measured using LPAD from tobacco leaves following injection with 0.1 (♦); 0.5 (□), and 1 (▴) mm SNP. Inset, NtACS expression in tobacco following the application of SNP. B, Expression of ACS in tobacco at different hours postinjection of wild-type tobacco with 1 mm SNP. A gene probe for the Arabidopsis ACS6 (At4g11280) was used because this exhibited high homology to stress-activated tobacco NtACS2. C, Ethylene generation in wild-type Samsun (▴) and in two replicate leaves (SH, 1 [▪] and SH, 2 [□]) at various hai with 1 mm SNP. Some of the data presented in Figure 5A have been published in conference proceedings (Mur et al., 2003).