Table VI.
The rate of GSH degradation in Landsberg wild-type and ggt1-1/ggt4-1 mutant plants
Ten-day-old liquid-cultured plants were supplemented with BSO at 1 mm and incubated for 8, 12, 16, or 20 h, then concentrations of thiols were determined. The means ± sd of five biological replicates are shown.
| Line | BSO | GSH | γ-EC | Cys |
|---|---|---|---|---|
| nmol g−1 fresh weight | ||||
| Landsberg wild type | 0 mm | 443 ± 43 | 10.0 ± 1.0 | 40 ± 8 |
| 1 mm, 8 h | 297 ± 24 | 8.6 ± 1.9 | 42 ± 10 | |
| 1 mm, 12 h | 197 ± 52 | 7.5 ± 2.0 | 69 ± 5 | |
| 1 mm, 16 h | 132 ± 19 | 7.4 ± 2.5 | 80 ± 10 | |
| 1 mm, 20 h | 113 ± 27 | 6.3 ± 0.9 | 105 ± 24 | |
| ggt1-1/ggt4-1 | 0 mm | 404 ± 49 | 13.4 ± 2.9 | 39 ± 9 |
| 1 mm, 8 h | 267 ± 73 | 9.2 ± 0.4 | 48 ± 10 | |
| 1 mm, 12 h | 217 ± 58 | 8.6 ± 1.9 | 71 ± 2 | |
| 1 mm, 16 h | 149 ± 23 | 8.6 ± 0.9 | 86 ± 14 | |
| 1 mm, 20 h | 105 ± 20 | 8.5 ± 1.1 | 116 ± 26 | |