Skip to main content
. 2008 Nov;148(3):1474–1486. doi: 10.1104/pp.108.118810

Figure 1.

Figure 1.

Growth of AtCCX3-expressing yeast strains in AP and YPD media with different cation concentrations. A, Growth of yeast strain wx1 (nhx1) transformed with vector (p2UGpd; crosses), AtNHX1 (squares), AtsCCX3 (triangles), AtCCX3 (black circles), or AtCCX4 (diamonds) in AP over time. B, OD at 48 h for yeast strain AXT3 (ena1∷HIS3∷ena4, nha1∷LEU2, nhx1∷TRP1, ura3-1) grown in AP with different concentrations of NaCl + 1 mm KCl. C, OD at 48 h for yeast strain wx1 (nhx1) transformed with vector (p2UGpd), AtNHX1, AtCCX3, AtsCCX3, or AtCCX4 in AP with various concentrations of KCl. D, OD at 48 h for yeast strains (Δsmf1 + Δsmf2) transformed with vector (p2UGpd; crosses), AtNHX1 (squares), AtsCCX3 (triangles), AtCCX3 (black circles), or AtsCAX2(black triangles) in AP over time grown in YPD with different concentrations of MnCl2. Yeast strains expressing the different plasmids (n = 8) were serial diluted 125-fold, and 10 μL was placed into 190 μL of liquid selection medium containing NaCl, KCl, or MnCl2.