Figure 9.
Cation content in young and mature leaves of AtCCX3-expressing tobacco plants. ICP analysis from tobacco plants expressing AtCCX3. Relative percentage differences in the averages of seven independent lines are shown in the graphs. A, ICP analysis of young leaves from 28-d-old tobacco plants. B, ICP analysis of mature leaves from 40-d-old tobacco plants. se is for the relative percentage difference of the means. se was calculated as (mean CCX3/mean vector) × [square root [((se CCX3)2/(mean CCX3)2) + ((se vector)2/(mean vector)2)]] × 100 (http://www.census.gov/acs/www/Downloads/ACS/PercChg.pdf). Concentrations of K+, Na+, and Mn2+ ions in young leaves were 41,852, 2,150, and 170 μg g−1 for plants expressing vector compared with 75,800, 11,904, and 158 μg g−1 for plants expressing 35S∷AtCCX3. Concentrations of K+, Na+, and Mn2+ ions in mature leaves was 50,644, 3,127, and 98 μg g−1 for plants expressing vector compared with 77,051, 6,181, and 201 μg g−1 for plants expressing 35S∷AtCCX3.