Figure 4.
Analysis of S-oxygenation activity of FMOGS-OX1 to FMOGS-OX5 for 4-MTB GSL. Spheroplasts of E. coli expressing empty vector were used as negative control. A, Negative control; B, assay of FMOGS-OX1; C, assay of FMOGS-OX2; D, assay of FMOGS-OX3; E, assay of FMOGS-OX4; F, assay of FMOGS-OX5. Ratio of 4-MTB:(4-MTB + 4-MSB) was calculated to represent the S-oxygenating activity. Significance of the difference between the ratio of 4-MTB:(4-MTB + 4-MSB) for FMOGS-OX versus the negative control was determined by ANOVA. For FMOGS-OX1 to FMOGS-OX4, P < 0.001; for FMOGS-OX5, P = 0.16.