Table II.
Altered GS-OX activity in the FMOGS-OX5 T-DNA mutant
GSL content in seeds and leaves was analyzed. All plants were segregants derived from a parental line heterozygous for the T-DNA knockout allele. MT:(MS + MT) represents the S-oxygenation activity for the conversion from MT GSL to MS GSL. This T-DNA mutant is in a wild-type Col-0 background.
| MT:(MS + MT) | Leaf Tissue
|
Seed Tissue
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Homozygous Knockout (na = 35)
|
Homozygous Wild Type (n = 29)
|
Pd | Homozygous Knockout (n = 29)
|
Homozygous Wild Type (n = 26)
|
P | |||||
| Meanb | sec | Mean | se | Mean | se | Mean | se | |||
| Propyl GSL (C3) | NDe | ND | 0.03 | 0.000 | 0.03 | 0.000 | NS | |||
| Butyl GSL (C4) | 0.20 | 0.000 | 0.20 | 0.000 | NSf | 0.80 | 0.010 | 0.79 | 0.010 | NS |
| Pentyl GSL (C5) | ND | ND | 0.91 | 0.010 | 0.90 | 0.010 | NS | |||
| Hexyl GSL (C6) | ND | ND | ND | ND | ||||||
| Heptyl GSL (C7) | 0.20 | 0.020 | 0.20 | 0.030 | NS | ND | ND | |||
| Octyl GSL (C8) | 0.27 | 0.040 | 0.28 | 0.050 | NS | 0.39 | 0.010 | 0.37 | 0.010 | <0.001 |
a
Number of individual lines measured per genotype class.
b
Mean value of MT:(MS + MT).
c
Standard error for the mean value.
d
P value for GSL differences between the two genotypes as determined by ANOVA.
e
Given GSL was not detectable; therefore, no statistical analyses were conducted.
f
Nonsignificant P values (P > 0.05).