Table III.

Altered GS-OX activity in the FMO_GS-OX4 T-DNA mutant

GSL content in seeds and leaves was analyzed. All plants were segregants derived from a parental line heterozygous for the T-DNA knockout allele. MT:(MS + MT) represents the S-oxygenation activity for the conversion from MT GSL to MS GSL. This T-DNA mutant is in a wild-type Col-0 background.

MT:(MS + MT)	Leaf Tissue					Seed Tissue
	Homozygous Knockout (na = 40)		Homozygous Wild Type (n = 37)		Pd	Homozygous Knockout (n = 23)		Homozygous Wild Type (n = 24)		P
	Meanb	sec	Mean	se		Mean	se	Mean	se
Propyl GSL (C3)	0.26	0.020	0.24	0.020	NSe	NDf		ND
Butyl GSL (C4)	0.41	0.020	0.39	0.020	NS	0.72	0.010	0.71	0.010	NS
Pentyl GSL (C5)	0.23	0.020	0.22	0.020	NS	0.79	0.020	0.76	0.020	NS
Hexyl GSL (C6)	ND		ND			ND		ND
Heptyl GSL (C7)	0.25	0.010	0.23	0.010	NS	0.62	0.020	0.58	0.030	NS
Octyl GSL (C8)	0.11	0.010	0.12	0.010	NS	0.32	0.010	0.31	0.010	NS

^aNumber of individual lines measured per genotype class.

^bMean value of MT:(MS + MT).

^cStandard error for the mean value.

^dP value for GSL differences between the two genotypes as determined by ANOVA.

^eNonsignificant P values (P > 0.05).

^fGiven GSL was not detectable; therefore, no statistical analyses were conducted.