Table IV.
Altered GS-OX activity in the FMOGS-OX3 T-DNA mutant
GSL content in seeds and leaves was analyzed. All plants were segregants derived from a parental line heterozygous for the T-DNA knockout allele. MT:(OH + MT) represents the S-oxygenation activity for the conversion from MT GSL to MS GSL. This T-DNA mutant is in a wild-type Ler background.
| MT:(OH + MT) | Leaf Tissue
|
Seed Tissue
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Homozygous Knockout (na = 21)
|
Homozygous Wild Type (n = 26)
|
Pd | Homozygous Knockout (n = 17)
|
Homozygous Wild Type (n = 19)
|
P | |||||
| Meanb | sec | Mean | se | Mean | se | Mean | se | |||
| Propyl GSL (C3) | 0.38 | 0.020 | 0.02 | 0.020 | <0.001 | 0.72 | 0.030 | 0.21 | 0.020 | <0.001 |
| Butyl GSL (C4) | NDe | ND | ND | ND | ||||||
| Pentyl GSL (C5) | ND | ND | ND | ND | ||||||
| Hexyl GSL (C6) | ND | ND | ND | ND | ||||||
| Heptyl GSL (C7) | ND | ND | 0.74 | 0.040 | 0.72 | 0.040 | NS | |||
| Octyl GSL (C8) | 0.11 | 0.010 | 0.09 | 0.010 | NSf | 0.64 | 0.030 | 0.56 | 0.030 | NS |
a
Number of individual lines measured per genotype class.
b
Mean value of MT:(MS + MT).
c
Standard error for the mean value.
d
P value for GSL differences between the two genotypes as determined by ANOVA.
e
ND, GSL was not detectable; therefore, no statistical analyses were conducted.
f
NS, Nonsignificant P values (P > 0.05).