Table VII.
Altered GS-OX activity in the FMOGS-OX4 overexpression lines
GSL content in seeds and leaves was analyzed. All plants were segregants derived from two independent T1 generation 35S∷FMOGS-OX4 lines. MT:(MS + MT) represents the S-oxygenation activity for the conversion from MT GSL to MS GSL.
| MT:(MS + MT) | Leaf Tissue
|
Seed Tissue
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
|
35S∷FMOGS-OX4 (na = 66)
|
Col-0 (n = 23)
|
Pdgene |
35S∷FMOGS-OX4 (n = 13)
|
Col-0 (n = 6)
|
Pgene | |||||
| Meanb | sec | Mean | se | Mean | se | Mean | se | |||
| Propyl GSL (C3) | 0.13 | 0.010 | 0.13 | 0.010 | NSe | ND | ND | |||
| Butyl GSL (C4) | 0.26 | 0.010 | 0.25 | 0.010 | 0.001 | 0.75 | 0.040 | 0.74 | 0.050 | 0.030 |
| Pentyl GSL (C5) | 0.22 | 0.010 | 0.22 | 0.010 | NS | ND | ND | |||
| Hexyl GSL (C6) | NDf | ND | ND | ND | ||||||
| Heptyl GSL (C7) | 0.26 | 0.010 | 0.25 | 0.010 | NS | 0.37 | 0.040 | 0.30 | 0.080 | NS |
| Octyl GSL (C8) | 0.14 | 0.010 | 0.14 | 0.010 | NS | 0.21 | 0.030 | 0.18 | 0.050 | NS |
a
Number of individual lines measured per genotype class.
b
Mean value of MT:(MS + MT).
c
Standard error for the mean value.
d
P value for GSL differences between the two genotypes as determined by ANOVA.
e
Nonsignificant P values (P > 0.05).
f
Given GSL was not detectable; therefore, no statistical analyses were conducted.