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Characterization of ROMT activity in planta using Agrobacterium-mediated transient transformation. Tobacco leaf sectors (150 mg fresh weight) expressing GFP (A), grapevine STS (B), or coexpressing STS and ROMT (C) were excised 48 h after Agrobacterium-mediated transformation. Stilbene content was analyzed using HPLC-DAD. Peak 1, Piceid; peak 2, resveratrol; peak 3, pterostilbene. Identity of peak 3 was confirmed by GC-MS analysis (data not shown).
