Table I.

Sequences (5′ to 3′) of primer combinations and annealing temperatures for the seven α-expansin genes studied using real-time RT-PCR

These seven α-expansin gene fragments were cloned from the internodes of the alpine and prairie ecotypes of S. longipes. For each gene, sequences were identical in both ecotypes. The primers listed against a particular gene were used at the annealing temperatures indicated for the measurement of the transcript abundance of that gene using real-time RT-PCR. GenBank accession numbers for these genes are EU840703 to EU840714, EU840720, and EU840721.

Gene	Forward Primer	Reverse Primer	Annealing Temperature
			°C
SlEXPA1	GGCCATGCCTATGTTCCTAA	CCCTTTGATGCTTACGCTCT	62
SlEXPA2	GCATTGTCCCTGTTGCTTTT	CCTTGTCCCTTTAACCCACA	62
SlEXPA3	GTACCATGCCGAAAACAAGG	ACCCCAGTAGCGACTCATTG	62
SlEXPA4	GCTGGAATTGTCCCAGTCTC	ACCCCAGTAGCTTGACATGG	65
SlEXPA5	CCCGTCCTCATTTCGACTTA	ACCCCAGTAACGACTCATGG	65
SlEXPA6	TGTGCGAGAAAAGGAGGAGT	GCCAATTTTGTCCCCAGTAA	62
SlEXPA7	TTTGAGCTTAAGTGCGCAGA	ATGGCCAAGTCAAAGTGAGG	65
Sl18S	CCGTTGCTCTGATGATTCATGA	GTTGATAGGGCAGAAATTTGAATGAT	62