Prolonged exposure to a low R:FR ratio bypasses FLC-mediated late flowering without lowering FLC expression, but FLC blocks acceleration of flowering in response to a transient far-red exposure. A and B, Flowering time in high R:FR conditions (white bars; R:FR approximately 5) and low R:FR conditions (gray bars; R:FR approximately 0.15). Plants were grown in LD cycles (16 h of light/8 h of dark). Error bars represent sd. C, Transcript levels in 7-d-old seedlings assayed by semiquantitative RT-PCR. Seeds were germinated in continuous white light for 5 d and then either left in these high R:FR conditions for 2 d (R:FR approximately 6) or exposed to far-red-enriched light for 2 d (R:FR approximately 0.04) prior to tissue collection. UBQ, UBIQUITIN loading control. D, Flowering time under high R:FR conditions (white bars, W), low R:FR conditions (dark gray bars, FR), or conditions of 5 d of low R:FR exposure followed by a return to high R:FR exposure (light gray bars, 5 d FR). Light sources were as in A and B.