Figure 6.

Measurement of AOX capacity in intact tissue slices (A) and isolated mitochondria (B) from 10-week-old wild-type and transgenic tubers with decreased PKc. Data show the average KCN-insensitive, SHAM-sensitive respiration in the absence (light gray bars) and presence (black bars) of pyruvate. Respiration rates were calculated from oxygen consumption rates measured using a Clark electrode in the presence of KCN (to inhibit the COX), before and after the addition of pyruvate (to stimulate the AOX), followed by SHAM (to inhibit the AOX and calculate residual respiration rates, which were subtracted to obtain KCN-sensitive, SHAM-insensitive respiration). Error bars represent ses (n = 12 tubers from six different plants per line [A] or four mitochondrial preparations per line, representing four independent tuber harvests [B]). *, Significant difference from wild-type levels (P < 0.05).