Table I.
Enzyme activities in tubers of transgenic plants with decreased PKc and wild type
Maximal catalytic activities were measured in 10-week-old tubers. Data show the mean and se (n = six tubers from six different plants per line).
| Enzyme | Enzyme Activity
|
|||||
|---|---|---|---|---|---|---|
| Wild Type | PKC-11 | PKC-26 | PKC-25 | PKC-6 | PKC-15 | |
| nmol min−1 mg protein−1 | ||||||
| Hexokinase | 23.6 ± 1.4 | 27.5 ± 0.7 | 25.9 ± 1.6 | 23.8 ± 2.1 | 21.3 ± 2.1 | 23.9 ± 1.3 |
| Fructokinase | 30.7 ± 2.3 | 35.4 ± 3.2 | 26.6 ± 2.9 | 22.6 ± 3.9 | 25.3 ± 3.1 | 26.7 ± 2.0 |
| Phosphoglucomutase | 1,255 ± 75 | 1,245 ± 48 | 1,364 ± 90 | 1,066 ± 113 | 1,037 ± 67 | 1,147 ± 62 |
| ATP-dependent phosphofructokinase | 15.2 ± 1.8 | 15.7 ± 0.3 | 13.2 ± 1.3 | 11.2 ± 1.9 | 12.3 ± 2.3 | 13.3 ± 1.4 |
| PEPC | 19.6 ± 1.0 | 21.4 ± 1.1 | 22.9 ± 1.5 | 22.2 ± 1.3 | 18.6 ± 1.1 | 20.4 ± 0.7 |
| NAD-malate dehydrogenase | 1,828 ± 202 | 1,982 ± 333 | 1,760 ± 342 | 1,418 ± 318 | 1,436 ± 292 | 2,072 ± 359 |
| NAD malic enzyme | 8.4 ± 0.5 | 8.2 ± 1.0 | 9.0 ± 0.7 | 9.2 ± 1.2 | 9.0 ± 0.2 | 8.3 ± 1.1 |
| PEP phosphatase | 107 ± 5 | 122 ± 13 | 127 ± 8 | 111 ± 10 | 114 ± 8 | 122 ± 12 |