Quantification of pollen tube germination rates in CSLD mutants. A, Quantification of the in vitro germination rate of pollen tubes from csld1-1, csld2-1, and csld4-1 compared with wild-type (WT) Col-0 and qrt. Note that pollen tube germination was normal in csld2-1. B, Quantification of the number of pollen tubes germinating from quartets. In csld4-1, the maximum number of pollen tubes germinating from a single quartet was two, whereas up to four tubes germinated from the qrt quartet. Error bars indicate se among three independent experiments with 80 samples each.