Figure 6.

Effect of DPI on the expression of elicitor-responsive genes. A, Arabidopsis seedlings were treated with water (control, white bars), OGs (light gray bars), DPI (dark gray bars), or OGs + DPI (black bars). H₂O₂ accumulation in the culture medium, expressed as μm g⁻¹ fresh weight, was measured at the indicated times (min). Values are means of three samples ± sd. Asterisks indicate statistically significant differences between control and OG-treated seedlings, according to Student's t test (*, P < 0.05; ***, P < 0.01). The experiment was repeated twice with similar results. B, Arabidopsis seedlings were treated with DPI, OGs alone, or in the presence of DPI (OG + DPI) for the indicated time (min). Gene expression was analyzed by semiquantitative RT-PCR, using the UBQ5 gene as internal standard. This experiment was repeated twice with similar results.